SIRPA antibody (FITC)
This Mouse Monoclonal antibody specifically detects SIRPA in FACS. It exhibits reactivity toward Rat.
Quick Overview for SIRPA antibody (FITC) (ABIN120469)
Target
See all SIRPA Antibodies
SIRPA
(Signal-Regulatory Protein alpha (SIRPA))
Reactivity
Rat
Host
Mouse
Clonality
Monoclonal
Conjugate
This SIRPA antibody is conjugated to FITC
Application
Flow Cytometry (FACS)
Clone
OX-41
-
-
Specificity
-
This antibody recognises rat CD172a, also known as Signal regulatory protein (SIRP). MRC OX-41 binds to a different epitope on SIRP than ED9.
-
Purification
-
Affinity chromatography on Protein G
-
Immunogen
-
Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with the NSO/U mouse myeloma cell line.
-
Isotype
-
IgG2a
-
-
-
Application Notes
-
Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
-
Restrictions
-
For Research Use only
-
-
Concentration
-
0.1 mg/mL
-
Buffer
-
PBS, pH 7.4 containing 0.09 % Sodium Azide and 1.0 % Bovine Serum Albumin
-
Preservative
-
Sodium azide
-
Precaution of Use
-
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Handling Advice
-
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
-
Storage
-
4 °C/-20 °C
-
Storage Comment
-
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
Target
-
SIRPA
(Signal-Regulatory Protein alpha (SIRPA))
-
Alternative Name
-
CD172a / SIRPA
-
Background
-
CD172a is expressed selectively by myeloid cells and by neurons.Synonyms: BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, MFR, MYD1, Macrophage fusion receptor, MyD-1 antigen, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, Signal-regulatory protein alpha-1, Sirp-alpha-2, Sirp-alpha-3, Tyrosine-protein phosphatase non-receptor type substrate 1, p84
-
Gene ID
-
25528
-
NCBI Accession
-
NP_037148
-
UniProt
-
P97710
-